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玉米基因组DNA提取测定与转植酸酶基因检测方法的研究.pdf87页
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答辩委员会对论文的评语 选题意义、文献综述、论文所取得的成果及水平、学
风和论文写作水平、论文的不足之处 ： 转基因作物安全性问题越来越受到社会的关注。论文以玉米转植 酸酶基因快速检测为研究内容，选题有实用价值。 论文确立了磁珠法获得纯度高、完整性好的基因组DNA有效方 法；建立了转植酸酶基因的LA御检测方法，通过条件优化弘检测 灵敏度达到30拷贝数，高于PCR的1000拷贝数。 该论文内容充实，书写规范，反映出论文作者具有扎实的专业基 础知识和较强的独立科研能力。 在论文答辩过程中表述清楚、回答问题正确。 经答辩委员会表决，一致同意通过毕业论文答辩，建议授予工学 硕士学位。 对学位论文水 优秀 良好 一般 较差 平的总体评价 √ 答辩委员会表决结果： 。 同意授予硕士学位箩票，不同意授予硕士学位矿 票， 弃权O票。根据投票结果，答辩委员会做出建议授予该同学 硕士学位的决议。 答辩委员会主席签字． 穗天^专 伊f／年门影日 北京化工大学位论文原创性声明 本人郑重声明： 所呈交的学位论文，是本人在导师的指导下，独立
进行研究工作所取得的成果。除文中已经注明引用的内容外，本论文不含
任何其他个人或集体已经发表或撰写过的作品成果。对本文的研究做出重
要贡献的个人和集体，均已在文中以明确方式标明。本人完全意识到本声
明的法律结果由本人承担。 作者签名：琦叠马竭 日期： 卫!l：s：马 关于论文使用授权的说明 学位论文作者完全了解北京化工大学有关保留和使用学位论文的规
定，即：研究生在校攻读学位期间论文工作的知识产权单位属北京化工大
学。学校有权保留并向国家有关部门或机构送交论文的复印件和磁
正在加载中，请稍后...Fink Usage
F.A.Q. - 5. Installing, Using and Maintaining Fink
about every package, including those that are still in the unstable
section. The dselect and apt-get tools on
the other hand only know about the packages available as precompiled
binary packages. Many packages are not available in precompiled form
through these tools for a variety of reasons. A package must be in the
"stable" section of the latest point release to be considered, and it
must pass additional checks for policy compliance as well as licensing
and patent restrictions.If you want to install a package that is not available via
dselect / apt-get, you have to compile it
from source using fink install packagename
Make sure you have the Developer Tools installed before you try this.
(If there is no installer for the Developer Tools in your
/Applications folder, you can get them from the
after free registration.) See also the question about unstable
has been created to assist with that.
indicated problems (including kernel panics
and infinite hangs during patching) when using Fink to compile
packages while anti-virus software is installed. You may need to
switch off any anti-virus software before using Fink.
(thanks to Rob Braun) , and you can run fink configure to
choose to search "Master" source mirrors so that Fink will
automatically look there.If this doesn't work, please let the package maintainer (available
from "fink describe packagename
") know that the
URL not all maintainers read the mailing lists all of the
time.To get a usable source, first try hunting around the remote site in
other directories for the same version of the source that Fink wants
(e.g. in an "old" directory). Keep in mind, though, that some remote
sites like to trash the old versions of their packages. If the
official site doesn't have it, then try a web search--sometimes there
are unofficial sites that have the tarball you want. Another place to
which is where Fink stores sourcefiles from packages that have been
released in binary form. If all of the above fail, then you might
consider posting on the
to ask if anybody has the old source available to
give you.Once you locate the proper source tarball, download it manually,
and then move the file into your Fink source location (i.e. for a
default Fink install, "sudo mv package-source.tar.gz
/sw/src/". Then use 'fink install packagename
' as normal.If you can't get the source file, then you'll have to wait for the
maintainer to deal with the problem. They may either post a link to
the old source, or update the .info and .patch files to use the newer
in the Users Guide. If /sw/sbin is there, but
there are other directories ahead of it (e.g.
/usr/local/bin), then you will either want to
reorder your PATH so that /sw/sbin is near the
beginning. Or if you really need the other directory to be before
and this former directory includes another install-info directory, then you'll want to temporarily rename this install-info subdirectory when you use Fink.
), then you can get a .deb file by running sudo
apt-get install --reinstall --download-only packagename
. Otherwise you can build one yourself by running fink
rebuild packagename
, but it won't install yet.Once you have a valid .deb file, then you can reconstitute the
file. First become root by using sudo -s (enter your
administrative user password if necessary), and then use the following
command:dpkg -c full-path-to-debfile | awk '{if ($6 == "./"){ print "/."; } \
else if (substr($6, length($6), 1) == "/")\
{print substr($6, 2, length($6) - 2); } \
else { print substr($6, 2, length($6) - 1);}}'\
& /sw/var/lib/dpkg/info/packagename.liste.g.dpkg -c /sw/fink/debs/libgnomeui2-dev_2.0.6-2_darwin-powerpc.deb | awk \
'{if ($6 == "./") { print "/."; } \
else if (substr($6, length($6), 1) == "/") \
{print substr($6, 2, length($6) - 2); } \
else { print substr($6, 2, length($6) - 1);}}' \
& /sw/var/lib/dpkg/info/libgnomeui2-dev.listWhat this does is to extract the contents of the .deb file, remove
everything but the filenames, and write these to the .list file.您所在位置： &
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探究式阅读在农村高中英语教学中应用.pdf96页
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摘要 二十一世纪，探究性学习成为主流学习方法，中国正在目睹一场教学和学习方法上
的变革。国家教育部2001年7月制定颁发的英语课程标准中明确指出要“利用图书馆、
网络等信息渠道尝试进行探究性阅读”，教育者要“培养学生进行探究性阅读教学的创
新实践"。美国的阅读研究小组对1980年以来的阅读研究作了文献研究，结果发现研究
多是教材和教法方面的，而且在过去的几十年，不论教材和教法如何调整，学生的阅读
能力没什么改变，这种现象的原因在于，长久以来，教育工作者和研究者把研究的重点
放在了教师的课堂教学和教材方面，而没有充分重视学生“如何学”。可是，教不等于
学。尤其在农村地区，填鸭式教育仍占主导地位，从而使得学生的学习效率相当低而挫
伤了学生学习的积极性。在教师一教材一学生构成的教学三角结构中，“学生的学"处
于相当重要甚至是中心地位。针对我国农村地区学生阅读理解力低下的实际情况，我们
把研究的重点由“如何教”和“教什么"转移到“如何学”上来，实现由“教师为中心’’
到“学生为中心"的转变，而探究性学习正是实现这一转变的科学方法之一。目前出版
和发表的书籍和论文有很多是关于探究性学习的，且多限于理论研究或数理化方面的研
究。然而，在中国，探究性学习用于提高语言方面的研究还有很大的提高空间，尤其用
于提高农村地区学生的英语阅读能力方面的研究几乎是一项空白。因此，此项研究对于 以在中国农村地区全面培养多层次、高素质、有创造力的人才显得尤为重要。本篇论文
通过实证性研究，旨在回答这样一个问题：探究性阅读对于提高农村高中学生英语阅读
正在加载中，请稍后...