载体基本信息
载体质粒图谱和多克隆位点信息
psPAX2 10703 bp
circular SYN
DEFINITION
This file is created by Vector NTI
Location/Qualifiers
/organism=&psPAX2&
/mol_type=&other DNA&
misc_feature
/label=&CAG_enhancer&
/label=&ORF frame 3&
complement(802..1533)
/label=&ORF frame 3&
complement(843..1346)
/label=&ORF frame 1&
misc_feature
/label=&pCAG_F_primer&
/label=&Gag_HIV (variant)&
/gene=&Gag_HIV (variant)&
/label=&ORF frame 1&
/label=&ORF frame 3&
misc_feature
/label=&cPPT&
/label=&ORF frame 1&
misc_feature
/label=&RRE&
misc_feature
/label=&HIV_Rev_NES&
terminator
/label=&rb_glob_PA_terminator&
complement()
/label=&M13_reverse_primer&
misc_feature
complement()
/label=&M13_pUC_rev_primer&
complement()
/label=&lac_promoter&
/label=&SV40_promoter&
rep_origin
/label=&SV40_origin&
misc_feature
/label=&SV40pro_F_primer&
terminator
/label=&SV40_PA_terminator&
misc_feature
/label=&EBV_rev_primer&
rep_origin
complement()
/label=&pBR322_origin&
complement()
/label=&Ampicillin&
/gene=&Ampicillin&
complement()
/label=&ORF frame 3&
complement(1)
/label=&AmpR_promoter&
1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcgggccg gccgcgttga
1741 cgcgcacggc aagaggcgag gggcggcgac tggtgagaga tgggtgcgag agcgtcagta
1801 ttaagcgggg gagaattaga tcgatgggaa aaaattcggt taaggccagg gggaaagaaa
1861 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
1921 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
1981 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
2041 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
2101 caaaacaaaa gtaagaaaaa agcacagcaa gcagcagctg acacaggaca cagcaatcag
2161 gtcagccaaa attaccctat agtgcagaac atccaggggc aaatggtaca tcaggccata
2221 tcacctagaa ctttaaatgc atgggtaaaa gtagtagaag agaaggcttt cagcccagaa
2281 gtgataccca tgttttcagc attatcagaa ggagccaccc cacaagattt aaacaccatg
2341 ctaaacacag tggggggaca tcaagcagcc atgcaaatgt taaaagagac catcaatgag
2401 gaagctgcag aatgggatag agtgcatcca gtgcatgcag ggcctattgc accaggccag
2461 atgagagaac caaggggaag tgacatagca ggaactacta gtacccttca ggaacaaata
2521 ggatggatga cacataatcc acctatccca gtaggagaaa tctataaaag atggataatc
2581 ctgggattaa ataaaatagt aagaatgtat agccctacca gcattctgga cataagacaa
2641 ggaccaaagg aaccctttag agactatgta gaccgattct ataaaactct aagagccgag
2701 caagcttcac aagaggtaaa aaattggatg acagaaacct tgttggtcca aaatgcgaac
2761 ccagattgta agactatttt aaaagcattg ggaccaggag cgacactaga agaaatgatg
2821 acagcatgtc agggagtggg gggacccggc cataaagcaa gagttttggc tgaagcaatg
2881 agccaagtaa caaatccagc taccataatg atacagaaag gcaattttag gaaccaaaga
2941 aagactgtta agtgtttcaa ttgtggcaaa gaagggcaca tagccaaaaa ttgcagggcc
3001 cctaggaaaa agggctgttg gaaatgtgga aaggaaggac accaaatgaa agattgtact
3061 gagagacagg ctaatttttt agggaagatc tggccttccc acaagggaag gccagggaat
3121 tttcttcaga gcagaccaga gccaacagcc ccaccagaag agagcttcag gtttggggaa
3181 gagacaacaa ctccctctca gaagcaggag ccgatagaca aggaactgta tcctttagct
3241 tccctcagat cactctttgg cagcgacccc tcgtcacaat aaagataggg gggcaattaa
3301 aggaagctct attagataca ggagcagatg atacagtatt agaagaaatg aatttgccag
3361 gaagatggaa accaaaaatg atagggggaa ttggaggttt tatcaaagta ggacagtatg
3421 atcagatact catagaaatc tgcggacata aagctatagg tacagtatta gtaggaccta
3481 cacctgtcaa cataattgga agaaatctgt tgactcagat tggctgcact ttaaattttc
3541 ccattagtcc tattgagact gtaccagtaa aattaaagcc aggaatggat ggcccaaaag
3601 ttaaacaatg gccattgaca gaagaaaaaa taaaagcatt agtagaaatt tgtacagaaa
3661 tggaaaagga aggaaaaatt tcaaaaattg ggcctgaaaa tccatacaat actccagtat
3721 ttgccataaa gaaaaaagac agtactaaat ggagaaaatt agtagatttc agagaactta
3781 ataagagaac tcaagatttc tgggaagttc aattaggaat accacatcct gcagggttaa
3841 aacagaaaaa atcagtaaca gtactggatg tgggcgatgc atatttttca gttcccttag
3901 ataaagactt caggaagtat actgcattta ccatacctag tataaacaat gagacaccag
3961 ggattagata tcagtacaat gtgcttccac agggatggaa aggatcacca gcaatattcc
4021 agtgtagcat gacaaaaatc ttagagcctt ttagaaaaca aaatccagac atagtcatct
4081 atcaatacat ggatgatttg tatgtaggat ctgacttaga aatagggcag catagaacaa
4141 aaatagagga actgagacaa catctgttga ggtggggatt taccacacca gacaaaaaac
4201 atcagaaaga acctccattc ctttggatgg gttatgaact ccatcctgat aaatggacag
4261 tacagcctat agtgctgcca gaaaaggaca gctggactgt caatgacata cagaaattag
4321 tgggaaaatt gaattgggca agtcagattt atgcagggat taaagtaagg caattatgta
4381 aacttcttag gggaaccaaa gcactaacag aagtagtacc actaacagaa gaagcagagc
4441 tagaactggc agaaaacagg gagattctaa aagaaccggt acatggagtg tattatgacc
4501 catcaaaaga cttaatagca gaaatacaga agcaggggca aggccaatgg acatatcaaa
4561 tttatcaaga gccatttaaa aatctgaaaa caggaaaata tgcaagaatg aagggtgccc
4621 acactaatga tgtgaaacaa ttaacagagg cagtacaaaa aatagccaca gaaagcatag
4681 taatatgggg aaagactcct aaatttaaat tacccataca aaaggaaaca tgggaagcat
4741 ggtggacaga gtattggcaa gccacctgga ttcctgagtg ggagtttgtc aatacccctc
4801 ccttagtgaa gttatggtac cagttagaga aagaacccat aataggagca gaaactttct
4861 atgtagatgg ggcagccaat agggaaacta aattaggaaa agcaggatat gtaactgaca
4921 gaggaagaca aaaagttgtc cccctaacgg acacaacaaa tcagaagact gagttacaag
4981 caattcatct agctttgcag gattcgggat tagaagtaaa catagtgaca gactcacaat
5041 atgcattggg aatcattcaa gcacaaccag ataagagtga atcagagtta gtcagtcaaa
5101 taatagagca gttaataaaa aaggaaaaag tctacctggc atgggtacca gcacacaaag
5161 gaattggagg aaatgaacaa gtagatgggt tggtcagtgc tggaatcagg aaagtactat
5221 ttttagatgg aatagataag gcccaagaag aacatgagaa atatcacagt aattggagag
5281 caatggctag tgattttaac ctaccacctg tagtagcaaa agaaatagta gccagctgtg
5341 ataaatgtca gctaaaaggg gaagccatgc atggacaagt agactgtagc ccaggaatat
5401 ggcagctaga ttgtacacat ttagaaggaa aagttatctt ggtagcagtt catgtagcca
5461 gtggatatat agaagcagaa gtaattccag cagagacagg gcaagaaaca gcatacttcc
5521 tcttaaaatt agcaggaaga tggccagtaa aaacagtaca tacagacaat ggcagcaatt
5581 tcaccagtac tacagttaag gccgcctgtt ggtgggcggg gatcaagcag gaatttggca
5641 ttccctacaa tccccaaagt caaggagtaa tagaatctat gaataaagaa ttaaagaaaa
5701 ttataggaca ggtaagagat caggctgaac atcttaagac agcagtacaa atggcagtat
5761 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
5821 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
5881 aaaattttcg ggtttattac agggacagca gagatccagt ttggaaagga ccagcaaagc
5941 tcctctggaa aggtgaaggg gcagtagtaa tacaagataa tagtgacata aaagtagtgc
6001 caagaagaaa agcaaagatc atcagggatt atggaaaaca gatggcaggt gatgattgtg
6061 tggcaagtag acaggatgag gattaacaca tggaattctg caacaactgc tgtttatcca
6121 tttcagaatt gggtgtcgac atagcagaat aggcgttact cgacagagga gagcaagaaa
6181 tggagccagt agatcctaga ctagagccct ggaagcatcc aggaagtcag cctaaaactg
6241 cttgtaccaa ttgctattgt aaaaagtgtt gctttcattg ccaagtttgt ttcatgacaa
6301 aagccttagg catctcctat ggcaggaaga agcggagaca gcgacgaaga gctcatcaga
6361 acagtcagac tcatcaagct tctctatcaa agcagtaagt agtacatgta atgcaaccta
6421 taatagtagc aatagtagca ttagtagtag caataataat agcaatagtt gtgtggtcca
6481 tagtaatcat agaatatagg aaaatggccg ctgatcttca gacctggagg aggagatatg
6541 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
6601 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
6661 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg
6721 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
6781 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
6841 ctccaagcaa gaatcctagc tgtggaaaga tacctaaagg atcaacagct cctagggatt
6901 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
6961 aataaatctc tggaacagat ctggaatcac acgacctgga tggagtggga cagagaaatt
7021 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
7081 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
7141 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
7201 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
7261 tcgtttcaga cccacctccc aatcccgagg ggacccgaca ggcccgaagg aatagaagaa
7321 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc cttggcactt
7381 atctgggacg atctgcggag cctgtgcctc ttcagctacc accgcttgag agacttactc
7441 ttgattgtaa cgaggattgt ggaacttctg ggacgcaggg ggtgggaagc cctcaaatat
7501 tggtggaatc tcctacaata ttggagtcag gagctaaaga atagtgctgt tagcttgctc
7561 aatgccacag ccatagcagt agctgagggg acagataggg ttatagaagt agtacaagga
7621 gcttgtagag ctattcgcca catacctaga agaataagac agggcttgga aaggattttg
7681 ctataagctc gaaacaaccg gtacctctag aactatagct agcagatctt tttccctctg
7741 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga
7801 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
7861 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat
7921 atgccatatg ctggctgcca tgaacaaagg tggctataaa gaggtcatca gtatatgaaa
7981 cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt
8041 tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt
8101 ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc
8161 ttatgaagat ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc
8221 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
8281 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
8341 cgctttccag tcgggaaacc tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc
8401 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
8461 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
8521 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagcta
8581 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
8641 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
8701 atcatgtctg gatccgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
8761 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
8821 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
8881 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
8941 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
9001 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
9061 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
9121 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta
9181 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
9241 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
9301 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
9361 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
9421 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
9481 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
9541 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
9601 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
9661 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
9721 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
9781 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
9841 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
9901 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
9961 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
10021 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
10081 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
10141 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
10201 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
10261 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
10321 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
10381 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
10441 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
10501 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
10561 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
10621 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
10681 atttccccga aaagtgccac ctg
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BIOFENG. 生物风 版权所有 Powered by Biofeng我的野蛮女友2psp下载 2010年psp3000价格 - 七丽女性网
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我的野蛮女友2psp下载 2010年psp3000价格
来源：七丽女性网
索尼psp3000虽然是08年上市的一款psp，但是目前它依然是一款性价比比较高的psp，随着我的野蛮女友2psp电影下载的流行，2010年的psp3000价格有开始有人关心了。
关于psp3000的待机时间问题：5个小时左右的游戏时间一直是PSP最难以回避的问题。实际上，在GC2008开展之前，美国FCC就已经流出PSP3000的检测报告了，报告中显示PSP3000的电池型号与PSP2000相同，均为PSP-S110，容量为1200mAh，同时报告中还披露了另外两种规格电池，其一是1800mAh大容量电池。作为PSP主机最新型号，PSP3000相比PSP2000可使用更长时间。 用来看电影我的野蛮女友2下载版绰绰有余了，2010年psp3000价格是1250元，是性价比很高的一款索尼psp。
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