El discurso del policial. Reconfiguración del género en la sociedad contemporánea. Nuevas formas y temas para nuevas subjetividades sociales
Mossello, Fabián Gabriel y Melana, Marcela Fabiana y Divito, Miriam Luján y Sarasa, Gabriela Yolanda y Camilloni, María Luz Alejandra y Giammarini, Gabriela Luján y Gregorio, Ximena Elizabeth y Rivarola, Daniela Susana
El discurso del policial. Reconfiguración del género en la sociedad contemporánea. Nuevas formas y temas para nuevas subjetividades sociales.
[Proyectos de Investigación]
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Biblioteca Marcela Verde
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按字母分类 ：Expression of surface antigen gene p1 fused with hspB of Coxiella burnetii in E.coli--《Chinese Journal of Zoonoses》2004年09期
Expression of surface antigen gene p1 fused with hspB of Coxiella burnetii in E.coli
feng, NIU Dong
sheng, WEN Bo
hai, QIU Ling, CHEN Mei
(Institute of Microbiology and Epidemiology, Academy of Military Medical Science,Beijing 100071,China)
To fuse the gene encoding the surface antigen P1 with hspB of Coxiella burnetii and to express the p1
hspB fusion gene in E. coli cells. The p1 and hspB fragment was amplified from C. burnetii genomic DNA by PCR and the gene fragments were ligated into the prokaryotic expression vector pQE30 to construct pQE30/p1 and pQE30/hspB, respectively. The p1 from pQE30/p1 was fused with hspB of pQE30/hspB to construct pQE30/p1
hspB. The expression of p1
hspB fusion gene of pQE30/p1
hspB was induced with IPTG in E. coli cells.The P1
HspB fusion protein was observed to be about 83 kDa in SDS
PAGE and 12.1% of total cell protein
the fusion protein was recognized by the serum from mice infected with C. burnetii in immunoblot assay.The p1
hspB fusion gene of pQE30/p1
hspB is expressed in E. coli cells by inducing of IPTG and the resulting P1
HspB fusion protein has ability to react to the antibodies to C. the P1
HspB may be a new candidate of subunit vaccines against Q fever.
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supports all the CNKI
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【Citations】
Chinese Journal Full-text Database
CHANG Wei-Hong (Center for Drug Evaluation, State Drug Administration, Beijing 100050, China);[J];Academic Journal of Second Military Medical U2002-10
(Institute of Burn Research, Southwestern Hospital, Third Military Medical University, Chongqing 400038, China);[J];Immunological J2001-S1
MENG Song\|Dong
2\ TIEN Po
1(Institute of Microbiology,The Chinese Academy of Sciences,Beijing\ 100080)
2(Harvard University,Boston,Massachusetts MA02115,USA);[J];CHINESE JOURNAL OF BIOTECHNOLOGY;2000-04
【Secondary Citations】
Chinese Journal Full-text Database
Hong (Center for Drug Evaluation, State Drug Adminstration, Beijing 100050, China);[J];Academic Journal of Second Military Medical U2002-10
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