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您要找的内容已被删除Establishment and Optimization of Genetic Transformation System of Lemon[Citrus limon(L.)Burm.f.]--《Hubei Agricultural Sciences》2014年09期
Establishment and Optimization of Genetic Transformation System of Lemon[Citrus limon(L.)Burm.f.]
TONG ZHE Li-WU Li-WANG Zhi-JING Ying-SUN Zhong-Institute of Fruit and Tea,Hubei Academy of Agricultural S
Using pCAMBIA1391Z as vector, the genetic transformation system of lemon [Citrus limon(L.) Burm.f.]with Agrobacterium-mediated by EHA105 was optimized. The results showed that concentration of plant hormones, inoculum density and co-cultivation time had significant effects on lemon regeneration and transformation. It was most conducive to the regeneration of adventitious buds lemon epicotyl by adding 1.0 mg / L 6-BA and 0.1 mg / L NAA in MT medium. The best inoculum density and co-cultivation time were 0.6(OD600 nm) and two days. For the adventitious bud regeneration of lemon, the most benifical screening concentration of hygromycin was 5.0 mg / L.
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